|
KMID : 1007520140230020523
|
|
Food Science and Biotechnology
2014 Volume.23 No. 2 p.523 ~ p.529
|
|
|
Fractionation of Nitrogenous Compounds from Water Extracts of Doenjang and Their Physiological Activities
|
|
Lee Sang-Hyun
Jung Jae-Han
Lee Hyun-A
Lee Hye-Sook
Jeong Hyun-Do
Cherl Jae
|
|
|
Abstract
|
|
|
|
Nitrogenous compounds were separated from a water extract of doenjang (WED), and the distribution of their molecular weights, and anti-inflammatory and angiotensin-I converting enzyme (ACE) inhibitory activities were investigated. WED was fractionated by ultrafiltration into 5 individual groups. The nitrogenous compound content in the 5 fractions were 1,062.1, 34.5, 24.2, and 29.9 mg% for WED I, WED II, WED III, and WED V, respectively. ACE inhibitory activity was widely observed in all the fractions, which suggest that many ACE inhibitory peptides with various molecular weights were present. However, the most potent ACE inhibition was observed in WED I with an IC50 value of 116.90±2.25 μg/mL. Potent inhibitory effects on the production of inflammatory mediators were observed in the extracts of doenjang with molecular weights >1 kDa (WED II, WED III, and WED V), as demonstrated by the inhibition of lipopolysaccharide induced NO production in macrophages.
|
|
|
KEYWORD
|
|
|
ACE inhibitory activity, NO inhibitory effect, doenjang, peptide, sequential separation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|